human colon cancer tissue microarray  (ZHUOLI IMAGING TECHNOLOGY CO LTD)

Journal: bioRxiv

Article Title: Antagonizing the serotonin receptor HTR2B drives antigen-specific cytotoxic T-cell responses and controls colorectal cancer growth

doi: 10.1101/2025.02.26.640476

Figure Lengend Snippet: TCGA-COAD data were analyzed. (A) Kaplan-Meier survival analysis of HTR 2B expression (derived from TCGA data) in 220 colon adenocarcinomas patient’s cohort. “HTR 2B -high” versus “HTR 2B -low” data were segregated based on the cohort’s top or bottom 25% with intratumoral HTR 2B expression. Mantel-cox log rank test. (B) Representative images of HTR 2B and Ki67 in the control (lamina propria) and lamina propria of colon adenocarcinoma (COAD) tissue. The original magnification is 200X, and the inset images are 600X. (C) Representative images of serotonin (5-HT), neurofilament-A (NF-A) in the control (lamina propria) and lamina propria of colon adenocarcinoma (COAD) tissue IHC. Original magnification 200X. (D) RNA-seq datasets (n=30 samples) were segregated into “HTR 2B -high” and “HTR 2B -low” (highest fifteen and lowest fifteen intra-tumoral HTR 2B expression), and the differential expression of selected genes was analyzed. (E) Principal component analysis (PCA) of “HTR 2B -high” versus “HTR 2B -low” datasets (n=15 patients/group). (F) Venn diagram showing numbers of mutually exclusive or shared genes in each group. (G) A volcano plot shows differentially expressed genes (DEGs) of “HTR 2B -high” versus “HTR 2B -low” datasets (n=15 patients/groups) are shown. (H) Heatmap statistics differentially expressed selected gene sets. (I) Gene ontology of altered biological processes. (J) Gene set enrichment analysis (GSEA) of the curated gene sets that affect the regulatory and effector functions of the T cells. (K) Intra-tumoral immune cell signatures were analyzed by deconvolution using TIMER2.0 of HTR 2B -high and -low datasets. (L) A TIDE computational analysis of the association between the tumor-infiltrating CD8 T cells (CTL) level (expression of CD8A, CD8B, GZMA, GZMB, and PRF1. Overall, patient survival with the intratumoral HTR 2B gene expression level. For each patient cohort, tumor samples were divided into HTR 2B -high (samples with HTR 2B expression one standard deviation above the average, shown in the left survival plot) and HTR 2B -low (remaining samples shown in the right survival plot) groups.

Article Snippet: The human colon adenocarcinoma tissue microarray (Novus Biological, Catalog # NBP2-78088) was stained with the antibodies and detected using chromogenic reagent DAB.

Techniques: Expressing, Derivative Assay, Control, RNA Sequencing, Gene Expression, Standard Deviation

Journal: bioRxiv

Article Title: TMIGD1, a putative tumor suppressor, induces G2-M cell cycle checkpoint arrest in colon cancer cells

doi: 10.1101/2020.06.06.138057

Figure Lengend Snippet: ( A ) Two human colorectal cancer tissue microarray slides consist of 172 tissue samples were stained with anti-TMIGD1 antibody. Staining was scored as 0 (negative, <5% cells positive), 1+ (6-25% cells positive), 2+ (26-50% cells positive), and 3+ (>50% cells positive). The tumor differentiation was graded morphologically (grade 1, 2 and 3 as well, moderately and poorly differentiated). ( B ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor grade. ( C ) The average staining intensities were compared using ANOVA with Tukey post-hoc test per tumor differentiation. ( D ) Western blot analysis of TMIGD1 expression in human renal and colorectal cancer cell lines. ( E ) qPCR analysis of TMIGD1 expression in CRC cell lines (HT29, HCT116 and RKO). Human kidney epithelial cells, HK2 was used as a positive control.

Article Snippet: Two human colorectal cancer tissue microarray slides (US Biomax, catalog numbers BC05012a and BC05118a) consist of 172 tissue samples (72 on BC05012a and 100 on BC05118a) were stained with anti-TMIGD1 antibody.

Techniques: Microarray, Staining, Western Blot, Expressing, Positive Control